Chromatografická analýza krevních lipidů u diabetiků typu 2 – Ing. Michaela Kulhánková

Anotace:

P.edm.tem diserta.ni prace s nazvem .Chromatograficka analyza krevnich lipid. u diabetik. typu 2?g bylo ov..eni spravnosti pou.ivanych metod pro m..eni spektra methylester. mastnych kyselin. Dale byl sledovan vliv mastnych kyselin na n.ktere enzymy, p.edev.im ?˘9-desaturazu, ktere vyznamn. ovliv.uji onemocn.ni diabetes mellitus 2. typu. K analyze byly pou.ity vzorky plazmy anonymnich pacient. za.azenych do 3 skupin (zdravi, st.edn. a t..ce nemocni) podle hodnot glykovaneho hemoglobinu. Vzorek krevni plazmy byl pomoci vakuove ultracentrifugace rozd.len na jednotlive frakce. Tyto frakce byly podrobeny separaci pomoci tenkovrstve chromatografie, esterifikaci pomoci acetylchloridu a vysledne methylestery mastnych kyselin byly analyzovany pomoci plynove chromatografie. …víceméně

Abstract:

Aim of the dissertation thesis "Chromatographic analysis of blood lipids in type 2 diabetics" was to verify the accuracy of the methods used for measuring of fatty acids methyl esters. The influence of fatty acids on some enzymes, especially Ä9-desaturase, which significantly affect the disease of type 2 diabetes mellitus, was also measured. For the analysis of plasma samples, three groups of patients by the values of glycated haemoglobin, were used. A sample of blood plasma was separated into single fractions using vacuum ultracentrifugation. These fractions were separated by thin-layer chromatography, esterified using acetyl chloride. The fatty acid methyl esters were analysed by gas chromatography. …víceméně