Novel electrochemical sensors for analysis of biological active compounds

HASSANPOUR, Soodabeh

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Theses wz3n3k

Novel electrochemical sensors for analysis of biological active compounds – Soodabeh HASSANPOUR

Soodabeh HASSANPOUR

Disertační práce

Novel electrochemical sensors for analysis of biological active compounds

Abstract:

Recently, medical organizations and research disciplines have switched to employing affordable biosensors. Biosensors have become more critical in drug development, drug identification, bio-medicine, food safety, security, protection, and ecological research. It has directly contributed to developing specialized and reliable diagnostic equipment that uses biological sensing components as biosensors. Different analytes, biological receptors, and transducer concepts are used in biosensors. In recent years, innovative sensing platforms have been developed employing screen-printed electrodes (SPEs), which are inexpensive, simple, and quick mass production through thick film technology. During my Ph.D., I concentrated on designing, constructing, and validating several SPE-based (bio)sensors to identify biologically active compounds important for healthcare applications, demonstrating their significant potential as a suitable sensing platform. I created (bio)sensors for a variety of analytes, from nucleic acid to small molecules, with specific modification strategies to endow the platforms with selectivity toward the species of interest. I developed disposable electrochemical (bio)sensors in two papers based on modifying with single-walled carbon nanotubes (SWCNT) and molecularly imprinted polymers. In the first case, SWCNT-modified SPCE as disposable electrochemical sensors was proposed for quick determination of dasatinib in pharmaceutical formulations, demonstrating an excellent boosting effect on the oxidation response of dasatinib. The sensor was able to monitor different dasatinib concentrations with a limit of detection of 0.06 M. In the second paper, the SPE surface was modified with poly tyrosine-chitosan and combined with a self-assembly surface molecular imprinting approach to develop horseradish peroxidase (HRP)-imprinted biosensor. Additionally, the molecularly imprinted electrochemical biosensor for determination of HRP was expanded for the detection of H2O2 as 7 enzymatic substrates. The HRP-imprinted biosensor was determined H2O2 and HRP with detection limits of 2.18 nM and 9.39 × 10-8 mg/ml (2.34 pM), respectively. The last paper was related to developing a DNA-based bio-assay for determining Haemophilus influenza through bioconjugation of citrate-capped silver nanoparticles with pDNA toward target sequences detection. The ultra-sensitive fabricated optical genosensor was detected the synthesized probe (SH-5-AAT TTT CCA ACT TTT TCA CCT GCA T-3') of Haemophilus influenza with great selectivity and sensitivity after hybridization with cDNA with the low limit of quantification of 1 zM (zeptomolar). Finally, the designed genosensor is a significant diagnostic strategy for detecting Haemophilus influenza with great selectivity. …více

Abstract:

Recently, medical organizations and research disciplines have switched to employing affordable biosensors. Biosensors have become more critical in drug development, drug identification, bio-medicine, food safety, security, protection, and ecological research. It has directly contributed to developing specialized and reliable diagnostic equipment that uses biological sensing components as biosensors. Different analytes, biological receptors, and transducer concepts are used in biosensors. In recent years, innovative sensing platforms have been developed employing screen-printed electrodes (SPEs), which are inexpensive, simple, and quick mass production through thick film technology. During my Ph.D., I concentrated on designing, constructing, and validating several SPE-based (bio)sensors to identify biologically active compounds important for healthcare applications, demonstrating their significant potential as a suitable sensing platform. I created (bio)sensors for a variety of analytes, from nucleic acid to small molecules, with specific modification strategies to endow the platforms with selectivity toward the species of interest. I developed disposable electrochemical (bio)sensors in two papers based on modifying with single-walled carbon nanotubes (SWCNT) and molecularly imprinted polymers. In the first case, SWCNT-modified SPCE as disposable electrochemical sensors was proposed for quick determination of dasatinib in pharmaceutical formulations, demonstrating an excellent boosting effect on the oxidation response of dasatinib. The sensor was able to monitor different dasatinib concentrations with a limit of detection of 0.06 M. In the second paper, the SPE surface was modified with poly tyrosine-chitosan and combined with a self-assembly surface molecular imprinting approach to develop horseradish peroxidase (HRP)-imprinted biosensor. Additionally, the molecularly imprinted electrochemical biosensor for determination of HRP was expanded for the detection of H2O2 as 7 enzymatic substrates. The HRP-imprinted biosensor was determined H2O2 and HRP with detection limits of 2.18 nM and 9.39 × 10-8 mg/ml (2.34 pM), respectively. The last paper was related to developing a DNA-based bio-assay for determining Haemophilus influenza through bioconjugation of citrate-capped silver nanoparticles with pDNA toward target sequences detection. The ultra-sensitive fabricated optical genosensor was detected the synthesized probe (SH-5-AAT TTT CCA ACT TTT TCA CCT GCA T-3') of Haemophilus influenza with great selectivity and sensitivity after hybridization with cDNA with the low limit of quantification of 1 zM (zeptomolar). Finally, the designed genosensor is a significant diagnostic strategy for detecting Haemophilus influenza with great selectivity. …více

Keywords

(Bio)sensors Screen-printed electrodes Disposable Dasatinib Single-walled carbon nanotubes Horseradish peroxidase Hydrogen peroxide Molecularly imprinted polymer Poly(tyrosine)-Chitosan Bioelectrocatalyst Genosensor Haemophilus influenza DNA bio-assay citrate capped silver nanoparticle

Jazyk práce: angličtina

Datum vytvoření / odevzdání či podání práce: 2. 6. 2023

Obhajoba závěrečné práce

Vedoucí: doc. RNDr. Jan Petr, Ph.D.

Citační záznam

Citovat tuto práci

Citace dle ISO 690:

HASSANPOUR, Soodabeh. \textit{Novel electrochemical sensors for analysis of biological active compounds}. Online. Disertační práce. Olomouc: Univerzita Palackého v Olomouci, Přírodovědecká fakulta. 2023. Dostupné z: https://theses.cz/id/wz3n3k/.

@PhdThesis{HASSANPOUR2023thesis,
AUTHOR = "HASSANPOUR, Soodabeh",
TITLE = "Novel electrochemical sensors for analysis of biological active compounds [online]",
YEAR = "2023 [cit. 2024-06-26]",
TYPE = "Disertační práce",
SCHOOL = "Univerzita Palackého v Olomouci, Přírodovědecká fakultaOlomouc",
NOTE = "SUPERVISOR: doc. RNDr. Jan Petr, Ph.D.",
URL = "https://theses.cz/id/wz3n3k/",
}

@PhdThesis{HASSANPOUR2023thesis,
AUTHOR = {HASSANPOUR, Soodabeh},
TITLE = {Novel electrochemical sensors for analysis of biological active compounds},
YEAR = {2023},
TYPE = {Disertační práce},
INSTITUTION = {Univerzita Palackého v Olomouci, Přírodovědecká fakulta},
LOCATION = {Olomouc},
SUPERVISOR = {doc. RNDr. Jan Petr, Ph.D.},
URL = {https://theses.cz/id/wz3n3k/},
URL_DATE = {2024-06-26},
}

{{Citace kvalifikační práce
 | příjmení = HASSANPOUR
 | jméno = Soodabeh
 | instituce = Univerzita Palackého v Olomouci, Přírodovědecká fakulta
 | titul = Novel electrochemical sensors for analysis of biological active compounds
 | url = https://theses.cz/id/wz3n3k/
 | typ práce = Disertační práce
 | vedoucí = doc. RNDr. Jan Petr, Ph.D.
 | rok = 2023
 | počet stran =
 | strany =
 | citace = 2024-06-26
 | poznámka =
 | jazyk = 
}}

Jak správně citovat práci

HASSANPOUR, Soodabeh. Novel electrochemical sensors for analysis of biological active compounds. Olomouc, 2023. disertační práce (Ph.D.). UNIVERZITA PALACKÉHO V OLOMOUCI. Přírodovědecká fakulta

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Instituce archivující a zpřístupňující práci: UNIVERZITA PALACKÉHO V OLOMOUCI, Přírodovědecká fakulta

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UNIVERZITA PALACKÉHO V OLOMOUCI

Přírodovědecká fakulta

Doktorský studijní program / obor:
Analytical Chemistry / Analytical Chemistry

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Theses wz3n3k wz3n3k/2

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thesis Final-Thesis_Presentation_small_booklet-Soodabeh_Hassanpo.pdf

MARKLOVÁ, E.

3. 6. 2023

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Novel electrochemical sensors for analysis of biological active compounds – Soodabeh HASSANPOUR

Soodabeh HASSANPOUR

Disertační práce

Novel electrochemical sensors for analysis of biological active compounds

Novel electrochemical sensors for analysis of biological active compounds

Abstract:

Abstract:

Keywords

Obhajoba závěrečné práce

Citační záznam

Citace dle ISO 690:

Jak správně citovat práci

Plný text práce

Obsah online archivu závěrečné práce

Jak jinak získat přístup k textu

UNIVERZITA PALACKÉHO V OLOMOUCI

Práce na příbuzné téma

Složky

Soubory

Co je jinak přidání souboru

Co je jinak další operace se soubory

Co je jinak pohled pro experty

Co je nové vyhledávání souborů

Co je nové rychlý přístup k souborům

Co se chystá