Use of in vitro cultures in Tacca integrifolia

Pavla Bryxová

Bachelor's thesis

Abstract:

Tacca integrifolia (Disocoreaceae) is a perennial ornamental and medicinal plant originating from tropical Asian regions. The objective of this thesis was an optimization of micropropagation in this species. Shoot proliferation was tested on MS medium (Murashige and Skoog, 1962) with different concentrations of plant growth regulators (PGRs). Zeatin and 6-benzylaminopurine (BAP) were used at concentrations 0.3-1.5 mg.l-1. In total, 9 treatments including control were tested. For in vitro rooting, naphthaleneacetic acid at concentrations 0.1 and 0.3 mg.l-1 was tested. The most effective medium for shoot proliferation was medium supplemented with 1.5 mg.l-1 BAP, providing 2.89 shoots per explant. The highest number of roots was achieved on medium with addition of 0.3 mg.l-1 NAA (3.40 roots per explant) with length 0.83 cm. Well-rooted plants were successfully transferred to the green house. The percentage of survival plants was 100%. Optimized protocol of micropropagation might be used as an effective tool to obtain sufficient number of genetically uniform plants of T. integrifolia in short time, for ornamental, medicinal and conservation purposes.

Keywords

Adventitious shoots, Dioscoreaceae, in vitro propagation, plant growth regulator, rooting, Tacca integrifolia

Language used: English

Date on which the thesis was submitted / produced: 11. 4. 2016

Thesis defence

• Supervisor: Ing. Iva Viehmannová, Ph.D.

Czech University of Life Sciences Prague

Faculty of Tropical AgriSciences

Bachelor programme / field:
Agricultural Specialization / Agriculture in Tropics and Subtropics