3D Reconstruction of Interphase Chromosomes

Mgr. Jan Hubený

Advanced ('rigorózní') thesis

Abstract:

The main contribution of the thesis is design and proposal of several robust segmentation methods suitable for analysis of image data acquired using confocal microscope. We will focus on the specific problem of reconstruction and segmentation of interphase chromosome territories. We describe the PDE-based nonlinear filters for image preprocessing and implicit active contour models for object reconstruction. Both these techniques are not widely used in image analysis in confocal microscopy because they are quite complex and their computation can be very slow when processing large three dimensional data. Nevertheless, we will describe three successful segmentation and reconstruction techniques, which use above mentioned methods. We will show, that one can overcome slow computation speed, which is the main disadvantage of PDE-based image processing techniques, by using fast and sophisticated numerical approximations and schemes. Last but not least we will discuss the performance of proposed reconstruction methods and we will compare their properties.

Keywords

3D reconstruction, interphase chromosomes, fast marching algorithm, nonlinear diffusion filtering, Chan-Vese algorithm, biomedical application

Language used: Czech

Date on which the thesis was submitted / produced: 5. 5. 2006

Thesis defence

• Date of defence: 5. 5. 2006

Masaryk University

Faculty of Informatics

Advanced ('rigorózní řízení') programme / field:
Informatics / Informatics