Příprava kardiomyogenních progenitorů

Bc. Zuzana Tomášiková

Bachelor's thesis

Preparation of cardiomyogenic progenitors

Abstract:

A damaged or dysfunctional myocardium can currently be treated only by transplantation of a new organ from a suitable donor. However, this one the approach has many disadvantages, starting with the long waiting time for the donor,through ethical aspects, resulting in inappropriate immunocompatibility of the donor organ and its rejection. The solution comes with working with cardiomyogenic progenitor cells. The aim is to prepare in huge quantities a cell culture that regenerates indefinitely and is able todifferentiate into cardiomyocytes, thus replacing the damaged ones. In this thesis the topic of cardiomyogenesis, cardiomyocytes and their progenitors - their discovery, signaling and in vitro expansion is approached.

Abstract:

Poškodený alebo nefunkčný myokard sa v súčasnej dobe dá liečiť len transplantáciou nového orgánu od vhodného darcu. Avšak tento prístup má mnoho nevýhod, počnúc dlhou čakacou dobou na darcu, cez etické aspekty, končiac nevhodnou imunokompatiblitou darcovského orgánu a jeho rejekcia. Riešenie prichádza s prácou s kardiomyogénnymi progenitormi. Cieľom je pripraviť v obrovských množstvách bunkovú kultúru, ktorá sa neobmedzene obnovuje a je schopná sa diferencovať na kardiomyocyty, a tak nahradiť tie poškodené. V tejto práci je priblížená téma kardiomyogenézy, kardiomyocytov a ich progenitorov – ich objavenie, signalizácia a in vitro expanzia.

Keywords

kardiomyogenéza, kardiomyocyty, kardiomyogénne progenitory, in vitro expanzia, MICP progenitor, iPSCs, transdiferenciácia, cardiomyogenesis, cardiomyocytes, cardiomyogenic progenitors, expansion in vitro, MICP progenitor cell, transdifferentiation

Language used: Slovak

Date on which the thesis was submitted / produced: 25. 5. 2021

Thesis defence

• Date of defence: 14. 7. 2021
• Supervisor: Mgr. Jiří Pacherník, Ph.D.
• Reader: Mgr. Lucia Binó, Ph.D.

Masaryk University

Faculty of Science

Bachelor programme / field:
Experimental Biology / Special Biology