Optimization of Single-molecule FRET Experiment to observe the Conformational Dynamics in Sec Translocon During Protein Transport. – Lorin LEHNER
Lorin LEHNER
Bachelor's thesis
Optimization of Single-molecule FRET Experiment to observe the Conformational Dynamics in Sec Translocon During Protein Transport.
Mechanism of energy conversion in ATP driven molecular machines: single-molecule observation of conformational changes in sec translocon during protein transport
Abstract:
In this thesis, single molecule experiments were optimized for the detection of conformational changes of the lateral gate in translocon. Fluorescence Correlation Spectroscopy was performed to find the best suited acceptor dye in single molecule Förster Resonance Energy Transfer (smFRET). The smFRET data was then analyzed by the open-source python notebook FRETbursts and the different fluorescence …moreAbstract:
In this thesis, single molecule experiments were optimized for the detection of conformational changes of the lateral gate in translocon. Fluorescence Correlation Spectroscopy was performed to find the best suited acceptor dye in single molecule Förster Resonance Energy Transfer (smFRET). The smFRET data was then analyzed by the open-source python notebook FRETbursts and the different fluorescence …more
Language used: Czech
Date on which the thesis was submitted / produced: 14. 12. 2023
Thesis defence
- Supervisor: Mgr. Tomáš Fessl, Ph.D.
Citation record
ISO 690-compliant citation record:
LEHNER, Lorin. \textit{Optimization of Single-molecule FRET Experiment to observe the Conformational Dynamics in Sec Translocon During Protein Transport.}. Online. Bachelor's thesis. České Budějovice: University of South Bohemia in České Budějovice, Faculty of Science. 2023. Available from: https://theses.cz/id/1gedwn/.
The right form of listing the thesis as a source quoted
LEHNER, Lorin. Optimization of Single-molecule FRET Experiment to observe the Conformational Dynamics in Sec Translocon During Protein Transport.. České Budějovice, 2023. bakalářská práce (Bc.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta
Full text of thesis
Contents of on-line thesis archive
Published in Theses:- světu
Other ways of accessing the text
Institution archiving the thesis and making it accessible: JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH, Přírodovědecká fakultaUNIVERSITY OF SOUTH BOHEMIA IN ČESKÉ BUDĚJOVICE
Faculty of ScienceBachelor programme / field:
Biochemistry / Biological Chemistry
Theses on a related topic
-
Optimization of Single-molecule FRET Experiments to observe the Conformational Dynamics in Sec Translocon During Protein Transport.
Lorin LEHNER -
Mechanism of Energy Conversion in ATP Driven Molecular Machines: Development of Single-molecule Techniques for Observation of Conformational Changes in Sec Translocon during Protein Transport
Edrin SULEJMANI -
Single-molecule fluorescence detection in molecular biology
Tomáš FESSL -
Dvouohnisková FCS ve výzkumu koloidů
Romana Chovancová -
Inverzní FCS ve výzkumu koloidních systémů
Veronika Richterová
Name
Posted by
Uploaded/Created
Rights
Folders
Files
Bulánová, L.
15/12/2023