Vývojová glykomika klíštěte Ixodes ricinus

RNDr. Jarmila ŠTĚRBOVÁ

Disertační práce

Abstract:

Ticks are increasingly important vectors of human and animal disease; however, the role of their glycans and glycoconjugates in host interaction, pathogen transmission and allergy remains insufficiently understood. This study describes the presence, localisation and origin of sialylated glycans in Ixodes ricinus ticks, their organs, and the tick cell line. Using a combination of microscopic techniques, mass spectrometry, and biochemical analyses, sialylated glycans were found to localise predominantly in salivary glands and the gut, suggesting that most sialylated structures are host-derived and are transported from the blood meal through the gut to the salivary glands. Quantitative analyses of sialic acid, including its metabolically incorporated analogues, further demonstrate that most of the sialylated molecules in adult ticks originate from host blood rather than de novo synthesis, a conclusion supported by similar observations in tick cell cultures. Despite earlier reports indicating minimal sialic acid presence in arthropods, genomic and biochemical analyses in this study identified active sialyltransferase genes and confirmed the tick's capacity to synthesise its own sialylated glycoproteins, further demonstrated through in vitro feeding experiments and Click chemistry-based confirming tick-origin sialylated glycoproteins in all life stages, from eggs and larvae to adult females.

Jazyk práce: angličtina

Datum vytvoření / odevzdání či podání práce: 21. 7. 2026

Obhajoba závěrečné práce

• Vedoucí: prof. RNDr. Libor Grubhoffer, CSc.

JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH

Přírodovědecká fakulta

Doktorský studijní program / obor:
Integrativní biologie / Integrativní biologie