In vitro propagation of Oxalis tuberosa

Martin Smetana

Bachelor's thesis

Abstract:

Oxalis tuberosa Molina (Oxalidaceae) is a tuber bearing crop originating from the Andean mountain range in South America. The aim of this thesis was to develop a protocol leading to fast and successful in vitro micropropagation of this crop species. For shoot formation MS medium (Murashige and Skoog 1962) with plant growth regulators at different concentrations was used. 6–Benzylaminopurine (BAP) and zeatin (ZEA) were used either separately at concentration 0.1 mg l-1, or in combination with α– Naphtaleneacetic acid (NAA) at concentration 0.03 mg l-1. As control, MS medium without plant growth regulators was used. For in vitro rooting, MS medium containing NAA at concentrations of 0.1 or 0.3 mg l-1 was used. The most effective medium for shoot formation was the one containing 0.1 mg l-1 BAP, providing 11.30 shoots per plant, forming the highest number of meristems. The shoots rooted on all tested media, however optimal medium for rooting was the control reaching approximately 14.00 roots per explant in mean length of 2.08 cm. The micropropagation protocol developed in this study might be used to obtain high amounts of plants of O. tuberosa in a relatively short time period, and it can be used as a supportive technique for conservation and breeding purposes in oca.

Keywords

Andean tuber crop, micropropagation, oca, plant growth regulator

Language used: English

Date on which the thesis was submitted / produced: 4. 5. 2020

Thesis defence

• Supervisor: Ing. Iva Viehmannová, Ph.D.

Czech University of Life Sciences Prague

Faculty of Tropical AgriSciences

Bachelor programme / field:
Agricultural Specialization / Agriculture in Tropics and Subtropics