Porovnanie imunoanalytickej a POCT metódy stanovenia troponínu a d-dimérov - význam pre akútnu medicínu

Bc. Jana Jurčová

Bachelor's thesis

Comparison of immunoanalytical and POCT methods of troponin and d-dimer determination and its significance for acute medicine

Abstract:

This bachelor´s thesis focuses on the comparison of various methods of determination of cardiac troponin T, I and d-dimers in patients using immunoanalytical and POCT methods. Troponins are determined in patients mainly in connection with suspected myocardial infarction. During the practical part of my thesis, determination of troponin T is carried on Roche Cobas 8000 analyzer and troponin I on Abbott Architect. The results were then compared to those obtained from POCT AQT90 FLEX analyzer. D-dimers are most often examined in the laboratory when there is a clinical sign of tromboembolic disease, particularly pulmonary embolism or deep venous thrombosis. In this thesis, a comparison between determination of d-dimers on STA R analyzer and AQT90 FLEX analyzer was performed.

Abstract:

Táto bakalárska práca sa zaoberá porovnaním imunoanalytickej a POCT metódy stanovenia troponínu a d-dimérov. Kardiálne troponíny sa u pacientov stanovujú hlavne v súvislosti s podozrením na infarkt myokardu. Troponín T bol stanovený na analyzátore Cobas 8000, troponín I na Abbott Architect i1000SR. D-diméry sa v laboratóriu najčastejšie vyšetrujú pri podozrení na pľúcnu embóliu alebo hlbokú žilovú trombózu. Na ich stanovenie bol použitý koagulačný analyzátor STA R. Analyzátor AQT90 FLEX bol použitý k POCT metóde na stanovenie troponínu I a d-dimérov.

Keywords

troponíny, d-diméry, imunochemické stanovenie, POCT, infarkt myokardu, pľúcna embólia, hlboká žilová trombóza troponin, d-dimer, immunochemical determination, myocardial infarction, pulmonary embolism, deep vein trombosis

Language used: Slovak

Date on which the thesis was submitted / produced: 29. 4. 2024

Thesis defence

• Date of defence: 11. 6. 2024
• Supervisor: MUDr. RNDr. Michal Řiháček, Ph.D.
• Reader: prof. MUDr. Dalibor Valík, Ph.D.

Masaryk University

Faculty of Medicine

Bachelor programme / field:
Medical Laboratory Technologist / Medical Laboratory Technologist