Analýza vybraných složek chmele s využitím opakované headspace extrakce

Bc. Iryna Shaparenko

Master's thesis

Analysis of selected hope components using multiple headspace extraction

Abstract:

Tato diplomova prace je zam..ena na analyzu vybranych slo.ek chmele vicenasobnou headspace mikroextrakci tuhou fazi, ve spojeni s plynovou chromatografii a hmotnostni detekci. V teoreticke .asti jsou popsany chmel a n.ktere jeho odr.dy, chmelove slo.ky, vliv t.chto slou.enin na lidske zdravi a jejich vyu.iti. Jsou zde popsany metoda MHS-SPME a kolorimetricke m..eni barevnosti potravin. V prakticke .asti byla nejprve provedena optimalizace metody pomoci volby vhodneho objemu vzorku a teploty extrakce. Dale je popsana kvalitativni analyza odr.d chmele pomoci GC-MS. Vysledkem byly chromatogramy s aroma profily chmelovych odr.d. Dale jsou prezentovany vysledky kvantitativni analyzy linaloolu, ?Ŕ-karyofylenu a karyofylen oxidu ve vybranych odr.dach chmele. Na konci je popsano m..eni barevnosti a porovnani vysledk. drcenych chmelovych granuli pomoci spektrofotometricke kolorimetrie.

Abstract:

This thesis is focused on the analysis of selected components of hops by multiple headspace solid phase microextraction, in connection with gas chromatography and mass detection. The theoretical part describes hops and some of their varieties, hop components, the effect of these compounds on human health and their use. The MHS-SPME method and colorimetric measurement of food color are described here. In the practical part, the optimization of the method was first carried out by choosing the suitable sample volume and extraction temperature. The qualitative analysis of hop varieties using GC-MS is also described. The result was chromatograms with aroma profiles of hop varieties. The results of the quantitative analysis of linalool, â-caryophyllene and caryophyllene oxide in selected hop varieties are also presented. At the end, the measurement of color and comparison of the results of crushed hop granules using spectrophotometric colorimetry is described.

Keywords

Chmel, MHS-SPME, GC-MS, m..eni barevnosti, linalool, ?Ŕ-karyofylen, karyofylen oxid

Language used: Czech

Date on which the thesis was submitted / produced: 25. 8. 2022

Accessible from:: 31. 12. 2999

Thesis defence

• Supervisor: doc. Ing. Martin Adam, Ph.D.

University of Pardubice

Faculty of Chemical Technology

Master programme / field:
Evaluation and Analysis of Foodstuffs / Evaluation and Analysis of Foodstuffs