Optimizing soil DNA extraction protocols to fit 3rd generation, single-strand sequencing technologies – Dmytro SHUMLIANSKYI
Dmytro SHUMLIANSKYI
Bachelor's thesis
Optimizing soil DNA extraction protocols to fit 3rd generation, single-strand sequencing technologies
The recent technologies in DNA sequencing like nanopore and PacBio allow direct sequencing of long single strands of DNA without amplification. This opens the path to a much improved environmental genome sequencing of bacteria, archaea and viruses. However, our DNA extraction methods have not been tested and updated for these new technologies.
Abstract:
Nucleic acid extraction is one of the basic procedures in molecular biology, allowing isolating of RNA and DNA from biological samples. This process, known as total nucleic acid extraction, enables various downstream applications encompassing gene expression analysis, genotyping, sequencing, and pathogen detection. However, current methods for generating high-quality and high-molecular-weight nucleic …moreAbstract:
Nucleic acid extraction is one of the basic procedures in molecular biology, allowing isolating of RNA and DNA from biological samples. This process, known as total nucleic acid extraction, enables various downstream applications encompassing gene expression analysis, genotyping, sequencing, and pathogen detection. However, current methods for generating high-quality and high-molecular-weight nucleic …more
Language used: English
Date on which the thesis was submitted / produced: 17. 8. 2023
Thesis defence
- Supervisor: MSc. Roey Angel, Dr. rer. nat.
Citation record
ISO 690-compliant citation record:
SHUMLIANSKYI, Dmytro. \textit{Optimizing soil DNA extraction protocols to fit 3rd generation, single-strand sequencing technologies}. Online. Bachelor's thesis. České Budějovice: University of South Bohemia in České Budějovice, Faculty of Science. 2023. Available from: https://theses.cz/id/44zasz/.
The right form of listing the thesis as a source quoted
SHUMLIANSKYI, Dmytro. Optimizing soil DNA extraction protocols to fit 3rd generation, single-strand sequencing technologies. České Budějovice, 2023. bakalářská práce (Bc.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta
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Institution archiving the thesis and making it accessible: JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH, Přírodovědecká fakultaUNIVERSITY OF SOUTH BOHEMIA IN ČESKÉ BUDĚJOVICE
Faculty of ScienceBachelor programme / field:
Biological Chemistry / Biological Chemistry
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