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Mgr. Eva Hasíková

Bachelor's thesis

Mutageneze rekombinantního proteinu L19R

Mutagenesis of recombinant gene L19R
Abstract:
Elicitiny reprezentují proteinové elicitory v interakci rostlina – patogen. Jsou schopné vázat steroly a vyšší mastné kyseliny. Kryptogein je 10 kDa protein vylučovaný oomycetou Phytophtora cryptogea, který způsobuje u rostlin tabáku silnou obranou reakci a může vést až k nekróze listů. U mutantní formy kryptogeinu L19R je aminokyselina leucin nahrazena argininem. Hlavním cílem práce bylo provést mutaci …more
Abstract:
Elicitins represent proteins elicitors in the plant-pathogen interactions. They are known to bind sterols and fatty acids. Cryptogein is 10 kDa elicitin secreted by oomycetes Phytophthora cryptogea, which induces strong defense reaction leading even to leaf necrosis in tobacco plants. L19R is a type of mutant cryptogein, where arginin is located instead of leucin. The main goal of this work was to …more
 

Keywords

mutagenesis cryptogein L19R Pichia pastoris
 
Language used: English
Date on which the thesis was submitted / produced: 15. 5. 2009
Identifier: https://is.muni.cz/th/qkd9u/

Thesis defence

  • Date of defence: 15. 6. 2009
  • Supervisor: doc. Mgr. Jan Lochman, Ph.D.

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Institution archiving the thesis and making it accessible: Masarykova univerzita, Přírodovědecká fakulta
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Masaryk University

Faculty of Science

Bachelor programme / field:
Biochemistry / Biochemistry

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