Atypická distribuce neuropeptidu corazoninu v buňkách kruhové žlázy u larev \kur{Drosophila melanogaster} s mutací v adipokinetickém hormonu

Bc. Kristýna SLÁDKOVÁ

Diplomová práce

Abstract:

Adipokinetic hormone (Akh) is an insect neuropeptide produced by the neuroendocrine gland of the corpora cardiaca and is involved in mobilization of energy stores and associated biochemical and physiological changes. In the Akh1 mutant line of the fruitfly, Drosophila melanogaster with a deletion of the second amino acid (Leu), an atypical localization of the neuropeptide corazonin (Crz) was observed. Crz is present in Akh-positive cells in the ring gland of the larval brain, indicating a possible representation of non-functional Akh1 by this phylogenetically related peptide. However, the connection of Crz expression in corpora cardiaca cells with a mutation in the receptor for Akh, Crz and sulphonylurea (i.e. components that are expected to be involved at different levels in the Akh signaling pathway) has not been demonstrated. Since this atypical distribution of Crz has not been confirmed in mutants with a null Akh mutation, the detected changes in the distribution of Crz in Akh1 mutants can be explained by the possible cross-reactivity of the anti-Crz antibody with Akh1 peptide. The possibility of cross-reactivity tested on a nitrocellulose membrane did not confirm this hypothesis, therefore the specific role of Crz in Akh1 mutants cannot be completely ruled out.

Jazyk práce: čeština

Datum vytvoření / odevzdání či podání práce: 22. 5. 2020

Obhajoba závěrečné práce

• Vedoucí: Mgr. Hana Sehadová, Ph.D.

JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH

Přírodovědecká fakulta

Magisterský studijní program / obor:
Biologie / Experimentální biologie - specializace Molekulární a buněčná biologie a genetika