Use of in vitro cultures in Incarvillea delavayi

Tomáš Hovorka

Bachelor's thesis

Abstract:

Incarvillea delavayi (Bignoniaceae) is a perennial herb with attractive pink flowers and it is used especially for ornamental purposes. The aim of this thesis was to optimize process of in vitro propagation in this ornamental species. As an initial plant material, seeds obtained through Index Seminum program were used. Two genotypes originated in Bratislava Botanical Garden (Slovakia), and one in Saint Galen Botanischer Garten (Switzerland) were used for the experiment. Seeds were surface sterilized using 70% ethanol and 1% NaClO than cultivated on half-strenght MS (Murashige a Skoog, 1962) medium until they germinated. For initial multiplication of plant material, the plants were repeatedly sub-cultivated on half-strenght MS medium with addition of cytokinin 6-benzylaminopurine (BAP) at concentration of 0.5 mg.l-1. For in vitro propagation experiment, BAP at concentrations 0.1 mg.l-1, 0.5 mg.l-1 and 1.0 mg.l-1 were tested. Medium (half-strenght MS medium) without the addition of PGRs was used as a control. Influence of genotype, BAP and influence of both concentration on shoot production was also tested. Root induction was tested using two concentrations (0.1 mg.l-1 and 0.3 mg.l-1) of 1-naphtalenacetic acid (NAA) in half-strenght MS medium. Results clearly showed that BAP and NAA at higher concentrations have significant influence on production of shoots and roots under in vitro conditions. Optimal shoot production (8.9 shoot per explant) was achieved on half-strenght MS medium with addition of 1.0 mg.l-1 BAP. Genotype and combination of genotype and BAP concentration have not significantly affected shoot production. The highest number of roots (9.8 root per explant) as well as their length (8.8) cm were achieved on medium supplemented with 0.3 mg.l-1 NAA. In this study, protocol for in vitro propagation and rooting in I. delavayi were optimized. The protocol might be used for large scale production in this species.

Keywords

Incarvillea delavayi, in vitro propagation, plant growth regulators, rooting, adventitious shoots

Jazyk práce: English

Datum vytvoření / odevzdání či podání práce: 13. 4. 2016

Obhajoba závěrečné práce

• Vedúci: Ing. Iva Viehmannová, Ph.D.

Czech University of Life Sciences Prague

Faculty of Tropical AgriSciences

Bachelor programme / odbor:
Agricultural Specialization / Agriculture in Tropics and Subtropics