Rozdíly v charakteristických vlastnostech a funkcích invariantních NKT buněk zdravých dárců

Bc. Karolína DOSTÁLOVÁ

Master's thesis

Anotácia:

iNKT cells are a subset of T cells, they express the same phenotypic markers usually found on T and natural killer (NK) cells. They comprise a lineage of CD1d-restricted glycolipid-reactive T lymphocytes which have an important role in imunity. They play critical role in the regulation of many different types of immune responses, ranging from self-tolerance and development of autoimmunity to responses to pathogens and tumors. These cells are gaining increasing importance in health care, particularly in hematooncology. In this study we examined the essential features and functions of Invariant Natural killer T cell (iNKT cells).iNKT cells were isolated from mononuclear cells (MNCs) of healthy donors. Then they were cultured under appropriate conditions in vitro. After reaching sufficient concentration and purity the iNKT cells were used for cytotoxic and immunomodulatory assays and immunophenotype determination. Our results showed that iNKT cells have varying qualities across donors and that they have a heterogeneous immunophenotype, cytotoxic and immunomodulatory potential.

Kľúčové slová

lymfocyt, invariantní NKT buňka, iNKT buňka, cytotoxický efekt, imunomodulace

Jazyk práce: Czech

Datum vytvoření / odevzdání či podání práce: 9. 12. 2019

Obhajoba závěrečné práce

• Vedúci: Mgr. Monika Holubová, Ph.D.

UNIVERSITY OF SOUTH BOHEMIA IN ČESKÉ BUDĚJOVICE

Faculty of Science

Master programme / odbor:
Biology / Clinical Biology