Izolace jednotlivých klonů evropských druhů Borrelie afzelii a Borrelie finlandensis a detekce plasmidů

Eva DRSKOVÁ

Bachelor's thesis

Isolation of individual clones from isolates of Borrelia afzelii and Borrelia finlandensis and the detection of their plasmid content

Abstract:

Lyme disease is a serious disease transmitted by ticks of the genus Ixodes. This disease is transmitted by the spirochete Borrelia burgdorferi. In Europe under the Borrelia burgdorferi sensu lato group are eighteen different species including Borrelia afzelii, Borrelia burgdorferi sensu stricto and Borrelia garinii which are known to be causative agents of the disease in humans. The genomes consist of a linear chromosome and a variable set of linear and circular plasmids. Some of these plasmids have been shown to be important for their infectious cycle in both the tick and animal. They are also known to be lost during in vitro passaging and during genetic manipulations. This project describes for the first time the use of plasmid specific PCR primers for the detection of plasmid content of individual clones, obtained by plating, from isolates of the European species Borrelia afzelii and Borrelia finlandensis.

Kľúčové slová

plasmidy, Lymeská borelióza, Borrelia burgdorferi, Borrelia afzelii, Borrelia finlandensis

Jazyk práce: Czech

Datum vytvoření / odevzdání či podání práce: 13. 12. 2013

Zverejniť od: 13. 12. 2013

Obhajoba závěrečné práce

• Vedúci: Ryan Rego, Ph.D.

UNIVERSITY OF SOUTH BOHEMIA IN ČESKÉ BUDĚJOVICE

Faculty of Science

Bachelor programme / odbor:
Biology / Biomedical Laboratory Techniques