Mechanismy působení nízkomolekulárních a vysokomolekulárních cyto-protektivních látek při vystavení hmyzího organismu nízkým teplotám.

Mgr. Petr HŮLA

Doctoral thesis

Anotácia:

Low temperatures limit the viability of cells, tissues and whole organisms. Temperate and polar insects overwinter with body temperatures below 0°C, sometimes with ice crystals in their hemolymph. Extracelular freezing is linked with cell osmotic dehydration. Thus, the freeze-tolerant insects had to evolve complex adaptation, including seasonal accumulation of low- and high-molecular substances, to protect their tissues against deleterious effects of cell freeze-dehydration. The mechanisms by which different substances exert their cryoprotective roles are theoretically diverse but practically poorly studied. In this thesis, I present results of metabolomic and transcriptomic analyses of putative cryoprotectants in the freeze-tolerant larvae of drosophilid fly, Chymomyza costata that accumulate in their hemolymph and tissues in response to cooling and drying. The functional aspects of low- (mainly proline and trehalose) and high- molecular substances (mainly LEA-like proteins) are considered.

Jazyk práce: Czech

Datum vytvoření / odevzdání či podání práce: 22. 11. 2022

Obhajoba závěrečné práce

• Vedúci: prof. Ing. Vladimír Košťál, CSc.

UNIVERSITY OF SOUTH BOHEMIA IN ČESKÉ BUDĚJOVICE

Faculty of Science

Doctoral programme / odbor:
Physiology and Immunology / Fyziologie a vývojová biologie