Molecular characterization of Plukenetia volubilis L. and analysis of seed storage protein pattern and protein fraction

Ing. Martin Ocelák

Doctoral thesis

Abstract:

In this study diversity and genetic relations in 173 Plukenetia volubilis L. samples were analysed by using ISSR markers. Thirty ISSR primers were used, only 8 showed variability in tested samples. ISSR fragments ranged from 200 to 2,500 bp. The obtained level of genetic variability was 36% among tested populations and 64% within populations. Although the diversity indexes were low, the cluster analysis revealed 8 clusters containing mainly samples belonging to individual populations. PCoA clearly distinguished Chumbaquihui, Pucallpa, Dos de Mayo, and Aguas de Oro populations, the others were intermixed. The obtained results indicated the level of genetic diversity is present in this location of Peru although it is influenced by anthropological aspects and independent on the geographical distances. The crude protein content of sacha inchi populations was detected at a range between 15.95% and 23.83% by the Kjeldahl method with mean at 20.61%. The protein fractions estimated by modified Osborne's method ranged between 10.2 and 18.4% for albumins-globulins; 0.1 and 0.5% for gliadins; and 3.6% and 8% for glutenins.

Keywords

ISSR, Molecular Markers, Plukenetia volubilis, Protein, Protein Fractions

Jazyk práce: English

Datum vytvoření / odevzdání či podání práce: 5. 9. 2016

Obhajoba závěrečné práce

• Vedúci: doc. Ing. Bohdan Lojka, Ph.D.
• Oponent: Pavel Vejl, doc. Dr. Ing., Tomáš Vyhnánek, externi

Czech University of Life Sciences Prague

Faculty of Tropical AgriSciences

Doctoral programme / odbor:
Agricultural Specialization / Tropical and Subtropical Agriculture