Studium interakcí mezi proteiny a RNA pomocí molekulové dynamiky

Bc. Josef Pasulka, Ph.D.

Master's thesis

Molecular dynamics study of protein and RNA interactions

Anotácia:

Pod pojmem RNA interference (RNAi) rozumime mechanismus genove regulace v pritomnosti dvousroubovicove RNA v bunce. Potlacovani RNAi proteiny p19 a B2 bylo studovano biochemickymi metodami. My chceme tuto problematiku protein-RNA komplexu studovat pomoci molekulove dynamiky a popsat pozorovane interakce.

Abstract:

RNA interference (RNAi) is an evolutionary conserved mechanism that regulates gene expression in response to the presence of double-stranded RNA (dsRNA) in the cell. The suppression of the RNAi by protein p19 and B2 was studied with biochemical experiments. We want to study the protein-RNA complexes by the molecular dynamics simulation and describe the observed interactions.

Keywords

double-stranded RNA, RNA-binding protein, RNA recognition, protein-RNA interaction

Jazyk práce: English

Datum vytvoření / odevzdání či podání práce: 23. 5. 2008

Obhajoba závěrečné práce

• Obhajoba proběhla 17. 6. 2008
• Vedúci: prof. RNDr. Jaroslav Koča, DrSc.

Masaryk University

Faculty of Science

Master programme / odbor:
Biochemistry / Biomolecular Chemistry