Development of microsatellite markers set for population genetic structure estimation of Peucedanum cervaria (Apiaceae)

Gabrielle Filippi

Diplomová práce

Abstract:

Phylogeography of dry grassland species remains a largely unexplored area of study. Yet these postglacial migration patterns are imperative to understanding the distribution of these highly valuable and under protected areas. The study species, Peucedanum cervaria (Apiaceae), is a characteristic species of Central European dry grassland ecosystems. In order to determine population genetic variability and history of P. cervaria in Central and Eastern Europe, six microsatellite markers specific to this species were developed. It has been determined, that there are several independent genetic lineages present in Central and Eastern Europe. On one side, there is a lineage specific to northern Central Europe that has a relationship to populations occurring in the Bohemian Massif. On the other side, there are lineages occurring exclusively in Carpathians, unique from all delimited lineages, including Czech ones. Finally, lineages occurring in the Pannonian Lowland are also present in the Czech Republic. Therefore, all analyzed Czech populations are derived from populations occurring in Pannonia, but those not from northern populations in fact represent an extension of the Pontic area.

Keywords

Microsatellites, Peucedanum cervaria, population genetic structure

Jazyk práce: angličtina

Datum vytvoření / odevzdání či podání práce: 18. 4. 2019

Obhajoba závěrečné práce

• Vedoucí: prof. Mgr. Bohumil Mandák, Ph.D.

Česká zemědělská univerzita v Praze

Fakulta životního prostředí

Magisterský studijní program / obor:
Inženýrská ekologie / Nature Conservation