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Bc. Ivo Fukala

Master's thesis

FnrP regulon bakterie Paracoccus denitrificans a jeho odezva na oxidační stres

The fnrP regulon of the bacterium Paracoccus denitrificans and its response to oxidative stress
Abstract:
Práce se zabývá vlivem transkripčního regulátoru FnrP na geny energetického metabolismu bakterie Paracoccus denitrificans. Studované geny byly vybrány na základě bioinformatické kontroly na přítomnost Fnr boxu. Výzkum proběhl především na úrovni transkripce pomocí RT-qPCR. Srovnáván byl divoký kmen a mutantní kmen s delecí FnrP, a to za normálních podmínek a prostředí oxidativního stresu, který by …more
Abstract:
The thesis is focused on the impact of the FnrP transcription regulator on energy metabolism-related genes of the Paracoccus denitrificans bacterium. The studied genes were chosen based on bioinformatic confirmation of presence of the Fnr-box. The research was performed mostly at the level of transcription using RT-qPCR. The wild-type strain was compared to FnrP-deficient mutant strain in normal conditions …more
 

Keywords

Paracoccus FNR FnrP PHB superoxid metylviologen
 
Language used: Czech
Date on which the thesis was submitted / produced: 13. 5. 2019
Identifier: https://is.muni.cz/th/v0pg2/

Thesis defence

  • Date of defence: 4. 6. 2019
  • Supervisor: prof. RNDr. Igor Kučera, DrSc.

Citation record

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Institution archiving the thesis and making it accessible: Masarykova univerzita, Přírodovědecká fakulta
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Masaryk University

Faculty of Science

Master programme / field:
Biochemistry / Biochemistry

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