Identification and Characterization of Some Viruses Infecting Grapevine, Cucurbitaceous Vegetable and Ornamental Plants

MSc. Karima Ben mansour

Disertační práce

Abstract:

The objective of this doctoral thesis was to utilise a range of bioinformatic tools to elucidate the evolutionary dynamics and genetic characterization of several plant viruses. This doctoral thesis comprises four distinct studies, the results of which have already been published. Two studies were conducted with the objective of elucidating the origin and evolutionary history of WMV (study 1) and GPGV (study 2). The results of our initial investigation indicate that WMV originated in East Asia more than 2,000 years ago. Before its global dispersal, the virus initially infected non-crop hosts, and subsequently transferred to a newly imported crop approximately 1000 years ago. The results of the second study confirmed that the virus originated in Asia, but probably further north and east, possibly in Japan or Korea. It also demonstrated that the virus diverged from GINV in wild grapevine before moving to Eurasian grapevine. Furthermore, two studies were conducted to investigate the prevalence and genetic characterization of viruses found in two plants, namely the grapevine (study 3). The grapevine plants under examination exhibited the presence of mixed infections, as well as several variants of the viruses in question within the same plant. Furthermore, the recombination pattern indicated a potential involvement of polyphagous insects in the transmission of GRVFV. Additionally, the second plant, myrobalan plum (study 4), was infected by a PNRSV isolate that was phylogenetically distinct from other isolates. Furthermore, a biological assay demonstrated that this isolate induces different symptom patterns than the common one.

Keywords

Molecular dating, Phylogenetic analysis, Recombination analysis, Comparative dating

Jazyk práce: angličtina

Datum vytvoření / odevzdání či podání práce: 24. 5. 2024

Obhajoba závěrečné práce

• Vedoucí: prof. Ing. Pavel Ryšánek, CSc.
• Oponent: Petr Svoboda, externi, Jaroslav Salava, CSc., Ing., externi, Dana Šafářová, externi

Česká zemědělská univerzita v Praze

Fakulta agrobiologie, potravinových a přírodních zdrojů

Doktorský studijní program / obor:
Agricultural Specialization / Agricultural and Forestry Phytopathology and Plant Protection