Aging-associated changes in the experssion of epigenetic factors and their impact on longevity in mammals

Vilja LANGER

Bakalářská práce

Abstract:

This thesis explores the epigenetic mechanisms potentially influencing ageing and longevity by analysing gene expression levels in kidney and liver tissues of mammals with varying life spans. This study builds on previous bioinformatics research, which established a correlation between epigenetic gene expression and age across species with different life spans, with the primary goal of validating these findings. The obtained results emphasise the possible link between epigenetic modifications and ageing processes using techniques such as RNA extraction, cDNA synthesis, and quantitative PCR. This work underscores the need for further research to understand how these genetic alterations could be targeted to manage age-related conditions and potentially extend life expectancy, thereby contributing to the broader field of genetics research.

Abstract:

This thesis explores the epigenetic mechanisms potentially influencing ageing and longevity by analysing gene expression levels in kidney and liver tissues of mammals with varying life spans. This study builds on previous bioinformatics research, which established a correlation between epigenetic gene expression and age across species with different life spans, with the primary goal of validating these findings. The obtained results emphasise the possible link between epigenetic modifications and ageing processes using techniques such as RNA extraction, cDNA synthesis, and quantitative PCR. This work underscores the need for further research to understand how these genetic alterations could be targeted to manage age-related conditions and potentially extend life expectancy, thereby contributing to the broader field of genetics research.

Keywords

epigenetics, age-related gene expression, longevity, short-lived mammals, quantitative PCR

Jazyk práce: angličtina

Datum vytvoření / odevzdání či podání práce: 19. 8. 2024

Obhajoba závěrečné práce

• Vedoucí: Mgr. Lenka Gahurová, Ph.D.

JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH

Přírodovědecká fakulta

Bakalářský studijní program / obor:
Biological Chemistry / Biological Chemistry