Identification and characterization of newly found antimicrobial peptide (IRAMP) from hard tick \kur{Ixodes ricinus}

Bc. Lucie OUŘEDNÍKOVÁ

Diplomová práce

Abstract:

Antimicrobial proteins (AMPs) are effector molecules and an important part of the innate immune system. AMPs have a broad antimicrobial spectrum and lyse microbial cells by interaction with biomembranes. Besides direct impact in host defence, AMPs are mediators of inflammation with impact on epithelial and inflammatory cells influencing diverse processes such as cytokine release, cell proliferation, angiogenesis, wound healing, chemotaxis, immune induction, and protease-antiprotease balance. AMPs could replace antibiotics which efficiency has decreased due to extensive clinical use. Therefore knowledge of mechanism of action of antimicrobial peptides, their properties and possible usage is essential for their further use as therapeutics. Ticks are blood-feeding ectoparisites that serve as extremely effective vectors of pathogens. Analysis of the ticks molecules that are involved in immune response to the pathogens invasion represent one of the strategies in searching for new compounds that might be used in future as theurapeutic agents. This study represents analysis of newly identified antimicrobial peptide form in the hard tick Ixodes ricinus (IRAMP). IRAMP revealed the high similarity to the recently described antimicrobial peptide isolated from hard tick Ixodes scapularis (protein ISAMP). Analysis and characterization of novel AMP, testing its antimicrobial potential and expression pattern are the main objectives of this study

Jazyk práce: angličtina

Datum vytvoření / odevzdání či podání práce: 14. 12. 2012

Zveřejnit od: 14. 12. 2012

Obhajoba závěrečné práce

• Vedoucí: Nataliia Rudenko, Ph.D.

JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH

Přírodovědecká fakulta

Magisterský studijní program / obor:
Biologie / Klinická biologie