Ektopická exprese kukuřičné β-glukosidázy Zm-p60.1 jako molekulární nástroj výzkumu subcelulární kompartmentace metabolismu cytokininů

Nagavalli Subbanna Kiran, M.Sc.

Doctoral thesis

Ectopic Over-expression of the Maize β-glukosidase Zm-p60.1 as a Molecular Tool to Probe Subcellular Compartmentation of Cytokinin Metabolism

Abstract:

This thesis deals with the subcellular compartmentation of cytokinin metabolism, specifically with the detailed characterization of the response of transgenic tobacco over-expressing the maize beta-glucosidase Zm-p60.1 in response to exogenous zeatin. Analyses were performed on two subcellular variants a) the wild-type enzyme where the enzyme is localised in the chloroplast and b) a recombinant version that is retargeted to the vacuole. The results obtained during the work on this thesis served to characterise the use of Zm-p60.1 as a powerful molecular tool to elucidate the complex network of regulation that underlies the subcellular compartmentation of cytokinin metabolism.

Keywords

beta-glukosidáza, chloroplast, cytokininovy metabolismus, ektopická exprese, konjugace hormonů, subcellulární kompartmentace, vakuole, zeatin, zeatin-O-glukosid. beta-glucosidase, cytokinin metabolism, ectopic expression, hormone conjugation, subcellular compartmentation, vacuole, zeatin-O-glucoside.

Language used: Czech

Date on which the thesis was submitted / produced: 26. 1. 2006

Thesis defence

• Date of defence: 26. 1. 2006
• Supervisor: prof. RNDr. Břetislav Brzobohatý, CSc.

Masaryk University

Faculty of Science

Doctoral programme / field:
Biology / Molecular and Cellular Biology