Differential gene expression as a tool to sort individual life stages of a mammalian parasite \kur{Trypanosoma brucei} – Bc. Michaela KUNZOVÁ
Bc. Michaela KUNZOVÁ
Master's thesis
Differential gene expression as a tool to sort individual life stages of a mammalian parasite \kur{Trypanosoma brucei}
Abstract:
This master thesis aimed to develop a system to sort individual life cycle stages of the mammalian parasite Trypanosoma brucei generated in vitro. T. brucei cell lines expressing developmentally regulatable fluorescent protein due to the stage specific 3'UTRs were generated. Furthermore, a double knock-out of a protein (TbIF1) presumably involved in the parasite's differentiation was attempted.Abstract:
This master thesis aimed to develop a system to sort individual life cycle stages of the mammalian parasite Trypanosoma brucei generated in vitro. T. brucei cell lines expressing developmentally regulatable fluorescent protein due to the stage specific 3'UTRs were generated. Furthermore, a double knock-out of a protein (TbIF1) presumably involved in the parasite's differentiation was attempted.
Language used: English
Date on which the thesis was submitted / produced: 17. 4. 2018
Thesis defence
- Supervisor: RNDr. Alena Zíková, Ph.D.
Citation record
ISO 690-compliant citation record:
KUNZOVÁ, Michaela. \textit{Differential gene expression as a tool to sort individual life stages of a mammalian parasite $\backslash$kur$\{$Trypanosoma brucei$\}$}. Online. Master's thesis. České Budějovice: University of South Bohemia in České Budějovice, Faculty of Science. 2018. Available from: https://theses.cz/id/9kaz3y/.
The right form of listing the thesis as a source quoted
KUNZOVÁ, Michaela. Differential gene expression as a tool to sort individual life stages of a mammalian parasite \kur{Trypanosoma brucei}. Č. Budějovice, 2018. diplomová práce (Mgr.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta
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Institution archiving the thesis and making it accessible: JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH, Přírodovědecká fakultaUNIVERSITY OF SOUTH BOHEMIA IN ČESKÉ BUDĚJOVICE
Faculty of ScienceMaster programme / field:
Biology / Experimental Biology
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