The Effect of external regulatory substances application on the progamic phase of male gametophyte development

Bc. Mohamed Noman

Master's thesis

Abstract:

Synthetic biostimulant Atonik is used to increase crop yield, especially in plants undergoing stress. The mechanisms in which it functions are not fully explored. An increase in pollen grain germination and tube growth leading to higher rate of fertilization and in turn seed and fruit formation is one possible mode of action. In this study the effect of Atonik on pollen germination and tube growth was tested using in vitro pollen cultures. Atonik was applied to SMM-MES medium in three concentrations of 0.1%, 0.2%, 0.5% and contrasted with a control variant. The cultures were cultivated for three time periods of 1 hour, 2 hours and 4 hours. After each time duration the germination rate and tube lengths of pollen grains was measured. The results after statistical analysis showed no significant effect of 0.1% and 0.2% Atonik on both pollen germination rate and pollen tube growth under normal or temperature stress conditions. 0.5% Atonik showed inhibitory effect on the pollen germination rate and pollen tube elongation with the inhibitory effect more prominent under colder temperatures. The assumptions stated in the hypothesis were refuted. The results obtained apply to in vitro pollen cultivation of tobacco pollen. Further study on in vivo effects of Atonik application is recommended.

Keywords

Nicotiana tabacum, pollen germination, pollen tube growth, in vitro, Atonik

Jazyk práce: English

Datum vytvoření / odevzdání či podání práce: 16. 4. 2019

Obhajoba závěrečné práce

• Vedúci: Ing. Petr Sedlák, Ph.D.
• Oponent: František Hnilička, Ph.D.

Czech University of Life Sciences Prague

Faculty of Agrobiology, Food and Natural Resources

Master programme / odbor:
Biotechnology / Biotechnology and Plant Breeding