Optimizing soil nucleic acid extraction protocol to fit third-generation, single-strand sequencing technologies

Dmytro SHUMLIANSKYI

Bakalářská práce

The recent technologies in DNA sequencing like nanopore and PacBio allow direct sequencing of long single strands of DNA without amplification. This opens the path to a much improved environmental genome sequencing of bacteria, archaea and viruses. However, our DNA extraction methods have not been tested and updated for these new technologies.

Abstract:

Nucleic acid extraction is one of the basic procedures in molecular biology, allowing isolating of RNA and DNA from biological samples. This process, known as total nucleic acid extraction, enables various downstream applications encompassing gene expression analysis, genotyping, sequencing, and pathogen detection. However, current methods for generating high-quality and high-molecular-weight nucleic acids have yet to keep pace with the requirements of modern third-generation sequencing methods. Therefore, we aim to improve the extraction protocol and create a fast, straightforward, and high-yield purification method for obtaining high-quality nucleic acids.

Abstract:

Nucleic acid extraction is one of the basic procedures in molecular biology, allowing isolating of RNA and DNA from biological samples. This process, known as total nucleic acid extraction, enables various downstream applications encompassing gene expression analysis, genotyping, sequencing, and pathogen detection. However, current methods for generating high-quality and high-molecular-weight nucleic acids have yet to keep pace with the requirements of modern third-generation sequencing methods. Therefore, we aim to improve the extraction protocol and create a fast, straightforward, and high-yield purification method for obtaining high-quality nucleic acids.

Keywords

Nucleic acid, extraction, third-generation sequencing, soil

Jazyk práce: angličtina

Datum vytvoření / odevzdání či podání práce: 15. 12. 2023

Obhajoba závěrečné práce

• Vedoucí: MSc. Roey Angel, Dr. rer. nat.

JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH

Přírodovědecká fakulta

Bakalářský studijní program / obor:
Biological Chemistry / Biological Chemistry