Single-molecule fluorescence detection in molecular biology

Mgr. Tomáš FESSL

Disertační práce

Anotace:

SMFD techniques offer genuine detection possibilities which are often inaccessible using ensemble methods. This was demonstrated in three projects investigating translocation activity of CHD4 protein, analysis of MS2 phage capsid assembly and in-cell characterization of DNA structure. In other projects, binding interactions between two fluorescent probes and a short oligonucleotide were characterized and all optical depth of focus extended microscope configuration for imaging of individual molecules inside bacterial cells was developed and tested.

Abstract:

SMFD techniques offer genuine detection possibilities which are often inaccessible using ensemble methods. This was demonstrated in three projects investigating translocation activity of CHD4 protein, analysis of MS2 phage capsid assembly and in-cell characterization of DNA structure. In other projects, binding interactions between two fluorescent probes and a short oligonucleotide were characterized and all optical depth of focus extended microscope configuration for imaging of individual molecules inside bacterial cells was developed and tested.

Klíčová slova

single-molecule fluorescence, FRET, DNA structure, macromolecular selfassembly, DNA-dye interaction

Jazyk práce: čeština

Datum vytvoření / odevzdání či podání práce: 16. 11. 2012

Zveřejnit od: 16. 11. 2012

Obhajoba závěrečné práce

• Obhajoba proběhla 15. 2. 2013
• Vedoucí: prof. RNDr. František Vácha, Ph.D.

JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH

Přírodovědecká fakulta

Doktorský studijní program / obor:
Biofyzika / Biofyzika