In vitro Induction of Polyploidy in Lycianthes asarifolia Bitter

Vera Zakamennaia

Bachelor's thesis

Abstract:

Lycianthes asarifolia (Kunth & Bouché) Bitter belongs to Solanaceae family. It is a fruiting plant origin from Latin America. The aim of this bachelor thesis was to obtain tetraploid plants (2n =4x=48) from original diploid (2n=2x=24), by in vitro induced polyploidy.For polyploidisation were used nodal segments that were exposed to the anti-mitotic agent oryzalin at concentrations of 20 µM, 40 µM, and 80 µM for 24 and 48 hours. These nodal segments were cultured on Murashige-Skoog (1962) medium without growth regulators. The ploidy level was detected by flow cytometry.In total were obtained 9 mixoploid plants, the most of after oryzalin treatment in 40 µM for 48 hours, also at 40 µM for 24 hours, and in concentration 80 µM for 24 and 48 hours. Tetraploid plants were not obtained. Whether a higher concentration of oryzalin or the use of another anti-mitotic agent is required for the induction of polyploidy in L. asarifolia.

Keywords

Lycianthes asarifolia, polyploidisaton, oryzalin, flow cytometry, micropropagation

Jazyk práce: English

Datum vytvoření / odevzdání či podání práce: 22. 5. 2020

Obhajoba závěrečné práce

• Vedúci: prof. Ing. Eloy Fernández Cusimamani, Ph.D.

Czech University of Life Sciences Prague

Faculty of Tropical AgriSciences

Bachelor programme / odbor:
Agricultural Specialization / Agriculture in Tropics and Subtropics