Orthohantaviruses in the reservoir and atypical hosts in the Czech Republic: spillover infection and indication of virus-specific tissue tropism

Mgr. Jan KAMIŠ

Rigorózní práce

Abstract:

Aim of this study was to reveal the presence of hantaviruses in natural reservoir rodent hosts in selected urban areas in the Czech Republic. Hantavirus rodent hosts were trapped, sampled and tested for hantavirus RNA in different tissues. Universal and specific primers for amplification of the large and medium fragments of hantavirus genomic RNA were used. Phylogenetic relationships were based on the obtained nucleotide sequences. Four different hantaviruses were detected, including two species pathogenic (or potentially pathogenic) for humans, further suggesting a threat for public health. Moreover, inter-family spillover infections and hantavirus species-associated tissue tropism were recorded in rodent hosts.

Keywords

Kurkino virus, Tula virus, Seewis virus, Asikkala virus, spillover infection, phylogeny, host specificity, tissue specificity, hantavirus

Jazyk práce: angličtina

Datum vytvoření / odevzdání či podání práce: 7. 9. 2022

Obhajoba závěrečné práce

JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH

Přírodovědecká fakulta

Rigorózní řízení / obor:
Biologie / Parazitologie