Purifikace, lyofilizace a měření aktivity lidských cholinesteráz

Bc. Trupti Kale

Diplomová práce

Purification, lyophilization and activity determination of human cholinesterases.

Abstract:

This diploma thesis is focused on the Purification, Lyophilization and Measurement of activity for Human Cholinesterase. The theoretical part describes basic information about choline receptors with emphasis given to butyrylcholinesterase. Butyrylcholinesterase plays a responsible role in cholinergic transmission and how its excessive production leads to certain neurological disorders. The later part is focused on role of butyrylcholinesterase as how it signifies in detoxifying action of certain drugs and foreign elements within the body systems. The practical aspects enlighten the areas of how the enzyme can be obtained and synthesized in laboratories under highly controlled conditions by various molecular and analytical techniques. All the crucial procedures were performed in laboratory as a part of experiment using specialized instruments that allowed for Purification, Lyophilization and Measurement of Activity for Butyryl cholinesterase as crude enzyme from recombinant hBChE from HEK293 cells. The confirmation of presence of enzyme and its purity level was confirmed by performing gel electrophoresis and western blotting.

Keywords

Butyryl cholinesterase, lyophilization, activity measurement, chromatography, protein concentration, gel electrophoresis, western blotting, purification

Jazyk práce: angličtina

Datum vytvoření / odevzdání či podání práce: 19. 7. 2023

Obhajoba závěrečné práce

• Obhajoba proběhla 22. 8. 2023
• Vedoucí: PharmDr. Adam Skarka, Ph.D.
• Oponent: PharmDr. Rudolf Andrýs, Ph.D.

Univerzita Hradec Králové

Přírodovědecká fakulta

Magisterský studijní program / obor:
Chemie / Toxicology and Analysis of Pollutants