Use of in vitro cultures in Grevillea banksii

Karolína Pumprová

Bachelor's thesis

Abstract:

Grevillea banksii (Proteaceae) is original Australian plant with very attractive flowers and a great economic potential as a cut flower on the global ornamental plant trade. The aim of this thesis was to optimize suitable protocol for rapid and effective in vitro micropropagation of this species. A propagation protocol was developed for establishment of in vitro culture, and multiplication and rooting of plants. Establishment in vitro was successfully provided by seeds, surface sterilised with 90% EtOH and 2% NaClO. For optimization of multiplication medium full-strength Murashige and Skoog (MS), half- strength Murashige and Skoog and Woody Plant Medium (WPM) media with various concentrations 0-1 mg/l of N6-benzylaminopurine (BAP) were used. Indole-3-butyric acid (IBA) was tested for root induction at concentrations 0.1 and 0.5 mg/l. After eight weeks of cultivation, from all tested types of media, MS medium supplemented with 0.1 BAP mg/l provided the highest length of plant (2.97 cm), number of buds per explant (7.4) and number of shoots per explant (1.75). Formation of roots was successful using both concentrations of IBA. Explants cultured on 0.5 IBA mg/l developed higher number of roots per explant (2.4). Rooting percentage in this treatment was 80% and thus, this medium can be recommended for rooting of this species. The process of propagation optimized within this thesis may be used for mass propagation of ornamental plant Grevillea banksii.

Keywords

in vitro propagation, plant growth regulator, Proteaceae, Grevillea, rooting

Language used: English

Date on which the thesis was submitted / produced: 15. 4. 2016

Thesis defence

• Supervisor: Ing. Iva Viehmannová, Ph.D.

Czech University of Life Sciences Prague

Faculty of Tropical AgriSciences

Bachelor programme / field:
Agricultural Specialization / Agriculture in Tropics and Subtropics