Studium a charakterizace komplexů siRNA - karbosilanových dendrimerů pro aplikace genové terapie.

Sofiya ROMANETS

Bachelor's thesis

The study and characterization of siRNA - carbosilane dendrimer complexes for gene therapy applications.

Abstract:

Cationic dendrimers are super branched polymers with positively charged vertex groups. With the help of these groups, the dendrimer interacts with the molecules of the biological system. The ability of cationic dendrimers to form complexes on the basis of electrostatic interactions ranks dendrimers among suitable non-viral vectors for the transfer of drugs, nucleic acids, and oligonucleotides, and for other possible uses in biomedicine. One nucleic acid that may be suitable for the generation of stable dendriplexes and then for transfection into cells is small interfering ribonucleic acid (siRNA). siRNA regulates cellular expression of genes by RNA interference and is thus one of the potential chances for treatment some of the genetic diseases. Carbosilane dendrimers are suitable candidates for transfer siRNA. Phosphonium carbosilane dendrimers demonstrably form dendriplexes with siRNA. These complexes are formed in different ratios with respect to the positive charge of the surface groups of the carbosilane dendrimer and the negative charge of the siRNA. The obtained dendriplexes of the individual charge ratios were characterized by several instrumental methods such as DLS, zeta potential, electrophoresis, etc.

Abstract:

Cationic dendrimers are super branched polymers with positively charged vertex groups. With the help of these groups, the dendrimer interacts with the molecules of the biological system. The ability of cationic dendrimers to form complexes on the basis of electrostatic interactions ranks dendrimers among suitable non-viral vectors for the transfer of drugs, nucleic acids, and oligonucleotides, and for other possible uses in biomedicine. One nucleic acid that may be suitable for the generation of stable dendriplexes and then for transfection into cells is small interfering ribonucleic acid (siRNA). siRNA regulates cellular expression of genes by RNA interference and is thus one of the potential chances for treatment some of the genetic diseases. Carbosilane dendrimers are suitable candidates for transfer siRNA. Phosphonium carbosilane dendrimers demonstrably form dendriplexes with siRNA. These complexes are formed in different ratios with respect to the positive charge of the surface groups of the carbosilane dendrimer and the negative charge of the siRNA. The obtained dendriplexes of the individual charge ratios were characterized by several instrumental methods such as DLS, zeta potential, electrophoresis, etc.

Keywords

Fosforiový karbosilanový dendrimer (s periferními substituenty P (Ph) 3), interference RNA, biomedicína, elektroforéza, DLS, zeta potenciál.

Language used: English

Date on which the thesis was submitted / produced: 3. 4. 2017

Thesis defence

• Supervisor: Mgr. Jan Malý, Ph.D.

JAN EVANGELISTA PURKYNĚ UNIVERSITY IN ÚSTÍ NAD LABEM

Faculty of Science

Bachelor programme / field:
Applied Physics / Applied Nanotechnology