Metodické a konceptuální důsledky přítomnosti polyfosfátů v buněčné biomase půdních mikroorganismů

Bc. Adéla TUPÁ

Master's thesis

Abstract:

The aim of this study was to determine whether soil microorganisms contain polyphosphates and whether they are quantifiable. To assess the presence of polyphosphates in soil microbial biomass, I utilized a combination of growth experiment conducted without the addition of phosphorus to the growth medium, measurement of stoichiometric changes in microbial biomass, and staining of polyphosphates by Neisser method. Furthermore, I employed a laboratory experiment to determine whether polyphosphates are detectable by the fumigation-extraction method. My results prove the presence of polyphosphates in the biomass of soil microorganisms and indicate that the polyphosphates are detectable by the fumigation-extraction method. Presence of polyphosphates have important implications for microbial phosphorus measurement and the development of biogeochemical models of phosphorus mineralization and immobilization in soil.

Kľúčové slová

Polyfosfáty, fumigačně-extakní metoda, ekologická stechiometrie, mikrobiální biomasa, půda

Jazyk práce: Czech

Datum vytvoření / odevzdání či podání práce: 12. 4. 2024

Obhajoba závěrečné práce

• Vedúci: RNDr. Petr Čapek, Ph.D.

UNIVERSITY OF SOUTH BOHEMIA IN ČESKÉ BUDĚJOVICE

Faculty of Science

Master programme / odbor:
Biology / Ecosystem Biology