Borrelia - host interactions: zoom in on the big picture.

Mgr. Martin STRNAD

Doctoral thesis

Abstract:

The thesis was written with the intention to bring together cutting-edge imaging methods and applications in order to illustrate how imaging can answer pathogenesis-related questions in Lyme disease at various resolution scale. Correlative light and electron microscopy, atomic force microscopy-based single-molecule force spectroscopy and solution nuclear magnetic resonance have been used to shed light on the underlying mechanisms associated with Lyme disease Borrelia infection. Specifically, the key molecular players and interactions responsible for the variance in the pathogenicity and disease outcome of Borrelia species have been studied. The rationale behind such studies was highlighted by review articles, which are part of the thesis.

Abstract:

The thesis was written with the intention to bring together cutting-edge imaging methods and applications in order to illustrate how imaging can answer pathogenesis-related questions in Lyme disease at various resolution scale. Correlative light and electron microscopy, atomic force microscopy-based single-molecule force spectroscopy and solution nuclear magnetic resonance have been used to shed light on the underlying mechanisms associated with Lyme disease Borrelia infection. Specifically, the key molecular players and interactions responsible for the variance in the pathogenicity and disease outcome of Borrelia species have been studied. The rationale behind such studies was highlighted by review articles, which are part of the thesis.

Keywords

microscopy, borrelia, diversity, lyme disease, adhesins

Language used: English

Date on which the thesis was submitted / produced: 15. 7. 2021

Thesis defence

• Date of defence: 22. 9. 2021
• Supervisor: Ryan Oliver Marino Rego, Ph.D.

UNIVERSITY OF SOUTH BOHEMIA IN ČESKÉ BUDĚJOVICE

Faculty of Science

Doctoral programme / field:
Molecular and Cell Biology / Molecular and Cell Biology and Genetics