Theses 

Transgenerational effect in \kur{Taraxacum brevicorniculatum}: test of a novel method of experimental plant DNA demethylation and its practical application in exploring the impact of maternal competition on progeny phenotype – Bc. Hana DVOŘÁKOVÁ

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JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH

Přírodovědecká fakulta

Magisterský studijní program / obor:
Botanika / Botanika

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Bc. Hana DVOŘÁKOVÁ

Diplomová práce

Transgenerational effect in \kur{Taraxacum brevicorniculatum}: test of a novel method of experimental plant DNA demethylation and its practical application in exploring the impact of maternal competition on progeny phenotype

Abstract: Spray application of 5­azacytidine on established plant seedlings was tested for its demethylating efficiency, as it represents a novel method for plant experimental demethylation with a potentially lower negative impact on plant development compared to the traditional application of the demethytaling agent through germination of seeds in its solution. Further, the 5­azacytidine spray application was used in practice to erase the epigenetic memory in offspring of Taraxacum brevicorniculatum plants from different competitive conditions. The impact of parental competition on the juvenile phenotype was estimated by measuring growth related traits, while the experimental demethylation allowed for evaluating the significance of DNA methylation marks in bioticaly induced transgenerational effects in T. brevirorniculatum.

Abstract: Spray application of 5­azacytidine on established plant seedlings was tested for its demethylating efficiency, as it represents a novel method for plant experimental demethylation with a potentially lower negative impact on plant development compared to the traditional application of the demethytaling agent through germination of seeds in its solution. Further, the 5­azacytidine spray application was used in practice to erase the epigenetic memory in offspring of Taraxacum brevicorniculatum plants from different competitive conditions. The impact of parental competition on the juvenile phenotype was estimated by measuring growth related traits, while the experimental demethylation allowed for evaluating the significance of DNA methylation marks in bioticaly induced transgenerational effects in T. brevirorniculatum.

Keywords: transgenerational effect, experimental DNA demethylation, 5-azacytidine, competition, seed mass

Jazyk práce: angličtina

  • Datum vytvoření / odevzdání či podání práce: 13. 12. 2016

Obhajoba závěrečné práce

  • Vedoucí: Francesco de Bello, Ph.D.

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DVOŘÁKOVÁ, Hana. Transgenerational effect in \kur{Taraxacum brevicorniculatum}: test of a novel method of experimental plant DNA demethylation and its practical application in exploring the impact of maternal competition on progeny phenotype. Č. Budějovice, 2016. diplomová práce (Mgr.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

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