Effects of newly synthesized compounds on the activity of intestinal microbial communities

Mgr. Vanina Nikolaeva

Master's thesis

Abstract:

The master’s thesis aims to determine the minimum inhibitory concentration of newly synthesized substances and their effect, either inhibitory or stimulating, on the physiological properties of selected bacterial genera. A minimum inhibitory concentration is the lowest concentration at which an antimicrobial agent inhibits the visible growth of a bacterial culture. The Proteobacteria phylum, more specifically family Enterobacteriacea, has been proven to be a major cause of inflammation in the human microbiome, therefore the antimicrobial compounds in this thesis are used against two representatives of this family – Escherichia coli CCM 3954 and Salmonella typhimurium LT 2-18. Additionally, the thesis has the goal of proposing the possible use of the antimicrobial agents displaying good results in the treatment of inflammatory bowel disease – a chronic condition, the prevalence of which has increased at an alarming rate during the past century.

Abstract:

The master’s thesis aims to determine the minimum inhibitory concentration of newly synthesized substances and their effect, either inhibitory or stimulating, on the physiological properties of selected bacterial genera. A minimum inhibitory concentration is the lowest concentration at which an antimicrobial agent inhibits the visible growth of a bacterial culture. The Proteobacteria phylum, more specifically family Enterobacteriacea, has been proven to be a major cause of inflammation in the human microbiome, therefore the antimicrobial compounds in this thesis are used against two representatives of this family – Escherichia coli CCM 3954 and Salmonella typhimurium LT 2-18. Additionally, the thesis has the goal of proposing the possible use of the antimicrobial agents displaying good results in the treatment of inflammatory bowel disease – a chronic condition, the prevalence of which has increased at an alarming rate during the past century.

Keywords

Minimální inhibiční koncentrace, enterobakterie, mikrobicidy, idiopatické střevní záněty, Crohnova nemoc, ulcerózní kolitida, mikrobiom, S. typhimurium, E. coli, Minimum inhibitory concentration, enterobacteria, antibacterial agents, inflammatory bowel diseases, Crohn’s disease, ulcerative colitis

Language used: English

Date on which the thesis was submitted / produced: 16. 6. 2023

Thesis defence

• Date of defence: 20. 6. 2023
• Supervisor: doc. Ivan Kushkevych, Ph.D.
• Reader: Josef Jampílek

Masaryk University

Faculty of Science

Master programme / field:
Molecular and Cell Biology / Molecular and Cell Biology