Reporterové geny - nástroj pro detekci genotoxicity

RNDr. Zuzana Flegrová, Ph.D.

Rigorózní práce

Reporter genes - tool for genotoxicity detection

Anotace:

viz. angl.

Abstract:

The RecA test, a luminescence SOS reporter test and two well-known SOS reporter assays, umuC test and SOS chromotest, were compared. The main point of this study was to indicate sensitivity of these tests. This all was on the basis of comparison of effects on standard mutagens and environmental samples. The results with the selected chemicals showed that the RecA test was less sensitive in a qualitative way in comparison to the umuC test, however its responses were quite comparable with the SOS chromotest. Results suggest that for our choice of environmental samples and chemical standards the UmuC test is optimal for genotoxicity detection in comparison to RecA test and SOS chromotest.

Keywords

reporter genes, SOS bacterial assays

Jazyk práce: angličtina

Datum vytvoření / odevzdání či podání práce: 11. 10. 2007

Obhajoba závěrečné práce

• Obhajoba proběhla 5. 12. 2007
• Oponent: doc. RNDr. Jakub Hofman, Ph.D., doc. RNDr. Kateřina Malachová, CSc.

Masarykova univerzita

Přírodovědecká fakulta

Rigorózní řízení / obor:
Biologie / Obecná biologie