Elucidating the source of bloodstream \kur{Trypanosoma brucei} mitochondrial ATP

Bc. Michaela HUSOVÁ

Master's thesis

Abstract:

For decades, it has been assumed that the reduced function and structure of the bloodstream form Trypanosoma brucei mitochondrion renders it a strictly ATP consuming organelle. Emerging evidence from refocused studies suggest that the bloodstream mitochondrion retains complex bioenergetic pathways that allow the parasite to adapt to various environments. This thesis is focused on the source of bloodstream mitochondrial ATP, with a special emphasis on the role of succinyl-CoA to produce ATP via mitochondrial substrate phosphorylation. We will also discuss alternative bioenergetic pathways present in this life stage of a human pathogen.

Abstract:

For decades, it has been assumed that the reduced function and structure of the bloodstream form Trypanosoma brucei mitochondrion renders it a strictly ATP consuming organelle. Emerging evidence from refocused studies suggest that the bloodstream mitochondrion retains complex bioenergetic pathways that allow the parasite to adapt to various environments. This thesis is focused on the source of bloodstream mitochondrial ATP, with a special emphasis on the role of succinyl-CoA to produce ATP via mitochondrial substrate phosphorylation. We will also discuss alternative bioenergetic pathways present in this life stage of a human pathogen.

Keywords

Trypanosoma brucei, mitochondria, bioenergetics, substrate phosphorylation, succinyl coenzyme A

Language used: English

Date on which the thesis was submitted / produced: 12. 4. 2021

Thesis defence

• Supervisor: RNDr. Alena Zíková, Ph.D.

UNIVERSITY OF SOUTH BOHEMIA IN ČESKÉ BUDĚJOVICE

Faculty of Science

Master programme / field:
Biology / Experimental Biology