Underutilized edible fruit tree species of the Philippines as a potential sources of antioxidants

Jana Tulková

Master's thesis

Abstract:

In present, human demand for plant-based product with health promoting effect has increased. Indigenous plant species, used in traditional cuisine and medicine has been frequently tested for antioxidant activity as a prevention of oxidative stress and subsequent diseases (cancer, anaemia, Parkinson disease). This thesis focuses on evaluation of in vitro antioxidant activity of underutilized fruit tree species growing and used in Philippine cuisine. For the assessment of antioxidant activity of methanol extracts, 2,2-diphenyl-1-picrylhydrazyl radical assay (DPPH), oxygen radical absorbance capacity (ORAC) assay were used and total phenolic content assay (TPC) was estimated. The strongest antioxidant activity possessed Stelechocarpus burahol with IC50 253.7 μg/mL (DPPH), 32.2 μg/mL (ORAC) and Broussonetia luzonica with IC50 90.9 μg/mL (DPPH), 37.9 μg/mL (ORAC). TPC was the highest at S. burahol with 72.2 mg/g and B. luzonica with GAE 133.0 mg/g. In scientific journals was not found any previous antioxidant activity evaluation of inflorescence B. luzonica. The fruit of S. burahol also was not tested, but in comparison to other plant parts tested in scientific articles, fruits of S. burahol possessed the strongest activity. S. burahol and B. luzonica have the potential for further practical application, assuming prior determination of their chemical composition. These species can contribute to species diversity of agricultural and forest composition and they are suitable for growing in multi-storey (B. luzonica) and Taungya (S. burahol) agroforestry systems.

Keywords

antioxidant activity, underutilized plants, DPPH, ORAC

Jazyk práce: English

Datum vytvoření / odevzdání či podání práce: 26. 4. 2019

Obhajoba závěrečné práce

• Vedúci: Ing. Johana Rondevaldová, Ph.D.
• Oponent: Jan Tauchen, Ph.D.

Czech University of Life Sciences Prague

Faculty of Tropical AgriSciences

Master programme / odbor:
Forestry Engineering / Tropical Forestry and Agroforestry