Allelic variation of HMW glutenin subunit in germplasm collection of Triticum spp.

B.Sc. Eric Mensah

Master's thesis

Abstract:

AbstractTriticum germplasm, a rich source of diversity of untapped alleles, are key for development of improved varieties. Researchers are seeking to unlock genetic variations for superior alleles with the use of markers. In view of this, biochemical markers were used to explore the genetic diversity of 159 wheat varieties of different geographical origins collected at the Genebank of Crop Research Institute Prague in the Czech Republic. High molecular weight glutenin subunits (HMW-GS) encoded at Glu-1 locus (Glu-A1, Glu-B1 and Glu-D1) were analysed and characterized for 147 of the varieties by one-dimensional SDS-PAGE analysis. Seed quality parameters (protein content, starch content, wet gluten content, gluten index and Zeleny sedimentation value) for all the 159 varieties were also determined. Total of 17 different subunits were recorded with 3, 8 and 6 on Glu-A1, Glu-B1 and Glu-D1 loci respectively. The two dominant alleles in each of the three loci were; Glu-A1 (null and 1), Glu-B1 (7+9 and 7+8) and Glu-D1 (5+10 and 2+12). Three unknown alleles were found on Glu-D1 locus. Combination of the alleles formed 39 different glutenin patterns with (0, 7+9, 2+12), (0, 7+9, 5+10) and (0, 7+8, 5+10) as the three most frequent ones. Ten varieties had Glu-score of 10. Analysis of Intra-varietal polymorphism revealed that 17.7% of the varieties were heterogenous with a greater percentage with Chinese origin. In the wheat collection analysed, protein content ranged from 10-19%. Wet gluten content ranged from 10-59%. Zeleny sedimentation value ranged from 12-61 ml with mean of 30.24 ml. Gluten index ranged from 6 and 100 respectively. Starch content ranged from 59-68%. The diversity observed in the analysed germplasm makes it valuable for development of improved varieties for multiple end-use baking products.

Keywords

Allelic variation, HMW-GS, SDS-PAGE, Wheat germplasm, Intra-varietal polymorphism, Seed quality parameters

Jazyk práce: English

Datum vytvoření / odevzdání či podání práce: 26. 4. 2018

Obhajoba závěrečné práce

• Vedúci: Ing. Iva Viehmannová, Ph.D.

Czech University of Life Sciences Prague

Faculty of Tropical AgriSciences

Master programme / odbor:
Tropical Agriculture / Tropical Crop Management and Ecology