Bordetella infections in animals and the role of type III secretion system in their virulence

Darya Kuzmenko

Bakalářská práce

Abstract:

This thesis investigates problematics of animal infections caused by genus Bordetella with special emphasis on members encoding type III secretion system (T3SS). Virulence of numerous pathogenic bacteria utilizes T3SS in terms of colonization, multiplication and persistence in the host. B. bronchiseptica has been known as an animal pathogen, precisely as a mammalian respiratory disease causative agent, since 1910. Over the second half of the 20th century, a lot of research was done on aetiology of the disease. An integrated prevention strategy, including vaccination and a set of zootechnical measures, was proposed. However, reports suggest that the bacteria still impose adverse infectious pressure on domestic animals. The goal of literature review was to inspect the field of contention and determine the T3SS role in veterinary infections provoked by Bordetella species. We begin with a brief portrayal of Bordetella genus and genus-associated diseases. It is followed by an assessment of the T3S apparatus general design, function and the way it enables different bacteria to establish infection. Finally, we summarise our current knowledge of the T3SS in Bordetella representatives. Additionally, the thesis includes a practical part which is divided into two sections. The first section aimed to discover so far unknown Bordetella T3SS substrates. By means of mass spectrometry analysis and comparison of secretion profile of wild type and T3SS-deficient ΔbscN mutant bacteria, we identified 14 proteins plausibly being secreted via the T3SS of B. bronchiseptica D445 (MO211). They were examined for presence of homologies and matched to the relevant loci in B. bronchiseptica D445 (MO211) genome. In the next section, we analysed and annotated B. bronchiseptica D445 (MO211) T3SS locus in agreement with generic standard rational nomenclature. The most intriguing outcome of this thesis is the detection of a small protein secreted in T3S-dependent manner and encoded within the T3SS locus. The protein was found to show sequence homology to members of YscX superfamily and thus might be hypothesised to play role in the process of the injectisome assembly.

Keywords

Bordetella bronchiseptica, type III secretion system, mass spectrometry, genome annotation

Jazyk práce: angličtina

Datum vytvoření / odevzdání či podání práce: 17. 4. 2019

Obhajoba závěrečné práce

• Vedoucí: Mgr. Barbora Černá Bolfíková, Ph.D.

Česká zemědělská univerzita v Praze

Fakulta tropického zemědělství

Bakalářský studijní program / obor:
Zemědělská specializace / Zemědělství tropů a subtropů