Mgr. Matej Fabišik

Bachelor's thesis

Analýza interakcí podjednotek SMC5-6 komplexu s DNA

Analysis of subunit interactions SMC5-6 complex with DNA
Abstract:
SMC5-6 complex belongs with cohesin and condensin to a family of SMC (Structure Maintenance of Chromosome) complexes whose role is to regulate a structure and dynamics of chromatin and participate in the reparation of the damaged DNA. We have managed to purify wild type and mutant protein dimer NSE1-NSE3 in this thesis. The EMSA method was used to prove that NSE1-NSE3 binds DNA. This bind is affected …more
Abstract:
SMC5-6 komplex patří spolu s kohezinem a kondenzinem do rodiny SMC (Structure Maintenance of Chromosome) komplexů, které mají za úkol regulovat strukturu a dynamiku chromatinu a podílet se na opravě poškozené DNA. V této práci se podařilo purifikovat přírodní i mutovaný proteinový dimer NSE1-NSE3. Metodou EMSA se podařilo prokázat, že protein NSE1-NSE3 váže DNA. Tato vazba je ovlivněná délkou DNA. …more
 

Keywords

SMC SMC5 SMC6 SMC5-6 complex NSE1 NSE3 DNA NSE1-NSE3
 
Language used: Slovak
Date on which the thesis was submitted / produced: 14. 5. 2012
Identifier: https://is.muni.cz/th/fl13q/

Thesis defence

  • Date of defence: 19. 6. 2012
  • Supervisor: doc. Mgr. Jan Paleček, Dr. rer. nat.

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