Morphological and molecular comparison between diploids and induced autotetraploids in Callisia fragrans Lindl (Woodson).

Bc. Kateřina Beranová

Diplomová práce

Abstract:

The objective of the current study was to determine morphological and molecular differences between diploids and induced autotetraploid plants in Callisia fragrans Lindl. (Woodson). Diploid plants were subjected to Murashige and Skoog (MS) medium containing oryzalin at concentrations 1, 5, and 10 μM for 4 and 8 weeks. Further, the ploidy levels of the plants were analyzed using flow cytometry and chromosome counting. Among all treatments, six tetraploid plants (2n = 4x = 24) were obtained after 8 weeks in MS medium containing 5 μM oryzalin. Upon successful establishment in the greenhouse, tetraploid plants were morphologically distinct compared to the diploid plants. The size of the leaf increased significantly and flower size nearly doubled when compared to the mother diploid plant. Inductively coupled plasma atomic emission spectroscopy was further utilized to compare the macro and micronutrients between diploid and tetraploid plants. Tetraploid plants exhibited significantly higher content of sodium, iron, and calcium. Interestingly, potassium content in the tetraploid was doubled. Molecular analysis utilizing iPBS and CDDP markers exhibited completely polymorphic profiles depicting variation between tetraploid and diploid genotypes. In C. fragrans, these molecular analyses were applied for the first time. In vitro polyploidization using oryzalin could effectively be used in inducing polyploids in this specie. Additionally, the results obtained in this study will provide a basis for future breeding opportunities in this species.

Keywords

Nutrient content, Commelinaceae, Medicinal plant, Molecular analysis

Jazyk práce: angličtina

Datum vytvoření / odevzdání či podání práce: 22. 4. 2022

Obhajoba závěrečné práce

• Vedoucí: prof. Ing. Eloy Fernández Cusimamani, Ph.D.

Česká zemědělská univerzita v Praze

Fakulta tropického zemědělství

Magisterský studijní program:
Tropical Crop Management and Ecology