Biochemické a fyziologické obranné reakce v těle včely medonosné ve stresový situacích

Bc. Nela PROKŮPKOVÁ

Master's thesis

Abstract:

This master thesis aimed to investigate the effects of honeybee venom and venom of the parasitoid wasp Habrobracon hebetor on the physiological characteristics of the honeybees Apis mellifera. The study compared the effects of the two venoms on various parameters. These involved measuring the levels of nutrients (carbohydrates, lipids) in hemolymph, the level and expression of adipokinetic hormone in the central nervous system, oxidative stress (in the gut, fat body), and the composition of honeybee venom of the worker honeybees A. mellifera. Furthermore, muscle structure was examined using transmission electron microscopy (TEM). The venoms were injected into the honeybee's body and the characteristics mentioned above were measured 24 hours after venom application. The results showed that the effects of H. hebetor venom on the nutrient levels in hemolymph varied - the levels of carbohydrates and lipids decreased and increased depending on the dose applied. The levels and expression of adipokinetic hormone in the central nervous system were reduced in both cases. Both venoms affected the composition of the venom but in different ways. The activity of oxidative stress enzymes was also affected by both venoms, where significant differences could be observed. The H. hebetor venom caused damage to the myofibril structure, while bee venom appeared to have no major effect on myofibrils.

Keywords

stres, včela medonosná, lumčík skladištní, jed, antistresová reakce

Language used: Czech

Date on which the thesis was submitted / produced: 12. 4. 2024

Thesis defence

• Supervisor: prof. RNDr. Dalibor Kodrík, CSc.

UNIVERSITY OF SOUTH BOHEMIA IN ČESKÉ BUDĚJOVICE

Faculty of Science

Master programme / field:
Molekulární a buněčná biologie a genetika / Molekulární a buněčná biologie a genetika