Real-time PCR a jeho využití v klinické molekulární diagnostice.

Bc. Lucie Lysková

Bachelor's thesis

Real - time PCR and his usage in clinical molecular diagnostics.

Abstract:

Real-time PCR je varianta PCR, která umožňuje přímou kvantifikaci PCR produktu v průběhu reakce, odtud název real time PCR- polymerázová řetězová reakce v reálném čase. Kvantifikace množství molekul nukleových kyselin je důležitá při detailním studiu genové exprese nebo diagnostice některých patogenů. Real time kvantitativní PCR je metoda, která zahrnuje jak amplifikaci tak analýzu, a u níž není potřeba desku s gelem, radioaktivitu, nebo vzorkovou manipulaci.

Abstract:

Real -time PCR is one of the PCR variant that allows direct quantification of PCR product continuously during the reaction. The quantification of DNA molecules amount plays the important role in the gene expression studying and in the diagnostics of some pathogens. The amplification steps and analysis are directly proceed in one reaction in one instrument during the quantitative real time PCR. There is no need to use radioactive labeling, gel board or any other manipulation with samples. The main aim of real time PCR system is fast and accurate recording of the PCR product continuously during the every amplification cycle of reaction. Real-time PCR monitors the fluorescence emitted during the reaction as an indicator of amplicon production during each PCR cycle (ie, in real time).

Keywords

Molekulární diagnostika nemocí, PCR, Real time PCR, Mutační analýza, Kvantitativní analýza

Language used: Czech

Date on which the thesis was submitted / produced: 15. 5. 2008

Thesis defence

• Date of defence: 4. 6. 2008
• Supervisor: RNDr. Iveta Valášková
• Reader: Mgr. Eva Flodrová

Masaryk University

Faculty of Medicine

Bachelor programme / field:
Specializations in Health Science / Laboratory Assistant