Optimization of CRISPR/Cas9 technique for the Ixodes ticks genome editing – Daniel KITZBERGER
Daniel KITZBERGER
Bachelor's thesis
Optimization of CRISPR/Cas9 technique for the Ixodes ticks genome editing
Optimization of CRISPR/Cas9 technique for the \kur{Ixodes} ticks genome editing
Abstract:
CRISPR/Cas9 technique was used for genome editing of several arthropod species, but it has not yet been used on Ixodes spp. tick species. Therefore, in my work, I performed a bioinformatic analysis of the I. ricinus transcriptome and I. scapularis genome to reveal the sequence of the target gene for the CRISPR/Cas9-mediated knock-out. We prepared single guide RNA and mixed it with the Cas9 endonuclease …moreAbstract:
CRISPR/Cas9 technique was used for genome editing of several arthropod species, but it has not yet been used on Ixodes spp. tick species. Therefore, in my work, I performed a bioinformatic analysis of the I. ricinus transcriptome and I. scapularis genome to reveal the sequence of the target gene for the CRISPR/Cas9-mediated knock-out. We prepared single guide RNA and mixed it with the Cas9 endonuclease …more
Language used: English
Date on which the thesis was submitted / produced: 9. 12. 2020
Thesis defence
- Supervisor: RNDr. Ján Štěrba, Ph.D.
Citation record
ISO 690-compliant citation record:
KITZBERGER, Daniel. \textit{Optimization of CRISPR/Cas9 technique for the Ixodes ticks genome editing}. Online. Bachelor's thesis. České Budějovice: University of South Bohemia in České Budějovice, Faculty of Science. 2020. Available from: https://theses.cz/id/p48vav/.
The right form of listing the thesis as a source quoted
KITZBERGER, Daniel. Optimization of CRISPR/Cas9 technique for the Ixodes ticks genome editing. Č. Budějovice, 2020. bakalářská práce (Bc.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta
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Institution archiving the thesis and making it accessible: JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH, Přírodovědecká fakultaUNIVERSITY OF SOUTH BOHEMIA IN ČESKÉ BUDĚJOVICE
Faculty of ScienceBachelor programme / field:
Biochemistry / Biological Chemistry
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