Optimization of CRISPR/Cas9 technique for the Ixodes ticks genome editing

Daniel KITZBERGER

Bachelor's thesis

Optimization of CRISPR/Cas9 technique for the \kur{Ixodes} ticks genome editing

Abstract:

CRISPR/Cas9 technique was used for genome editing of several arthropod species, but it has not yet been used on Ixodes spp. tick species. Therefore, in my work, I performed a bioinformatic analysis of the I. ricinus transcriptome and I. scapularis genome to reveal the sequence of the target gene for the CRISPR/Cas9-mediated knock-out. We prepared single guide RNA and mixed it with the Cas9 endonuclease to produce the Ribonucleoprotein complex and cleave the target sequence of the I. ricinus gene beta-galactoside alpha-2,6-sialyltransferase.

Abstract:

CRISPR/Cas9 technique was used for genome editing of several arthropod species, but it has not yet been used on Ixodes spp. tick species. Therefore, in my work, I performed a bioinformatic analysis of the I. ricinus transcriptome and I. scapularis genome to reveal the sequence of the target gene for the CRISPR/Cas9-mediated knock-out. We prepared single guide RNA and mixed it with the Cas9 endonuclease to produce the Ribonucleoprotein complex and cleave the target sequence of the I. ricinus gene beta-galactoside alpha-2,6-sialyltransferase.

Keywords

CRISPR/Cas9, Ixodes spp. ticks, genome editing, CRISPR

Jazyk práce: English

Datum vytvoření / odevzdání či podání práce: 9. 12. 2020

Obhajoba závěrečné práce

• Vedúci: RNDr. Ján Štěrba, Ph.D.

UNIVERSITY OF SOUTH BOHEMIA IN ČESKÉ BUDĚJOVICE

Faculty of Science

Bachelor programme / odbor:
Biochemistry / Biological Chemistry