CpSAT-1, a transcribed satellite sequence from the codling moth,\kur{Cydia pomonella\kur{}}

Mgr. Martina DALÍKOVÁ

Rigorózní práce

Abstract:

Satellite DNA represents one of the major components of repetitive genome components. Recent research is bringing more and more evidence about importance of this type of DNA, which can play both structural and functional roles in eukaryotic genomes. In this study, we described newly discovered satellite DNA in Cydia pomonella, CpSAT-1. Although this satDNA can be found in all chromosomes, it is surprisingly underrepresented on the W chromosome, the only heterochromatin block in Cydia pomonella genome. The CpSAT-1 is transcribed in all tested developmental stages and tissues. However, the potentially functional motives were found predominantly in non-conserved parts of monomer sequence. Thus the function of this new satellite DNA remains unknown.

Abstract:

Satellite DNA represents one of the major components of repetitive genome components. Recent research is bringing more and more evidence about importance of this type of DNA, which can play both structural and functional roles in eukaryotic genomes. In this study, we described newly discovered satellite DNA in Cydia pomonella, CpSAT-1. Although this satDNA can be found in all chromosomes, it is surprisingly underrepresented on the W chromosome, the only heterochromatin block in Cydia pomonella genome. The CpSAT-1 is transcribed in all tested developmental stages and tissues. However, the potentially functional motives were found predominantly in non-conserved parts of monomer sequence. Thus the function of this new satellite DNA remains unknown.

Keywords

satellite DNA, sex chromosomes, codling moth, expression, Cydia pomonella

Jazyk práce: angličtina

Datum vytvoření / odevzdání či podání práce: 10. 6. 2017

Obhajoba závěrečné práce

• Obhajoba proběhla 24. 7. 2017

JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH

Přírodovědecká fakulta

Rigorózní řízení / obor:
Biologie / Experimentální biologie